Jenkins to run ansible playbook to manage remote host

Question

I have installed Jenkins and Ansible in same EC2 machine (ubuntu). My docker machine is in centos. When I run ansible from ubuntu through terminal it is connecting to centos and is working. But when I try to run same ansible playbook from Jenkins pipeline it is throwing error

fatal: [W1]: UNREACHABLE! => {"changed": false, "msg": "Failed to connect to the host via ssh: Load key \"/home/ubuntu/EC2Tutorial.pem\": Permission denied\r\nec2-user@3.144.238.158: Permission denied (publickey,gssapi-keyex,gssapi-with-mic).", "unreachable": true}

Jenkins script
stage('Install Python 3') {
           steps {
                ansiblePlaybook credentialsId: 'test-server', installation: 'ansible', inventory: 'servers.inv', playbook: 'python3-playbook.yml'
           }
        } 

Ansible host

W1 ansible_host=3.144.238.158  ansible_user=ec2-user ansible_ssh_private_key_file=/home/ubuntu/EC2tutorial.pem

 Also host_key_checking = False added in ansible.cfg

Can you please guide me on this ?